Upon successful completion of this course, students should be able to: Understand Basic Principles Explain the fundamental concepts and principles of tissue and cell culture, including aseptic techniques, media preparation,…

Learning Outcomes By the end of the course, students are expected to be able to: 1.Explain the various cell lines used in mammalian tissue culture and their origins and uses…

A well-equipped and carefully organized laboratory is crucial for successful cell and tissue culture work. Proper planning ensures sterility, minimizes contamination, and facilitates efficient workflow. 1. Laboratory Setup Key Considerations…

Cell culture involves the growth of cells under controlled conditions, typically outside their natural environment. The main types of cell cultures can be classified based on the origin of the…

Culture media and reagents are essential components in microbiology, cell biology, and molecular biology, providing the nutrients, conditions, and environment required for the growth, maintenance, and study of microorganisms, cells,…

Sterile techniques are critical in cell culture to prevent contamination from microorganisms (bacteria, fungi, and viruses) and cross-contamination between cell lines. Contaminants can compromise experimental results and harm the integrity…

Cell isolation and preparation are fundamental steps in various biological and biomedical research areas, including virology, immunology, and cell biology. Obtaining cells for cell culture typically involves isolating cells from…

Cell culture is a fundamental technique in biological research, particularly in virology, immunology, molecular biology, and pharmacology. It involves growing and maintaining cells in a controlled artificial environment. 1. Types…

Tissue culture is a laboratory technique used to grow, maintain, or propagate cells, tissues, or organs in an artificial medium under sterile conditions. It is widely used in research and…