Topic 7: The Biology of Replication of Viruses

A. VIDEOS 

1. Viral replication process
2. The Infectious cycle
3. Attachment, entry and uncoating
4. Reverse Transcription and Integration
5. Viral DNA replication
6. DNA virus replication strategies
7. Life cycle for different types of DNA genomes
8. Replication of RNA viruses
9. RNA viruses replication strategies
10. Replication of ds RNA and ssRNA genomes
11. RNA Directed RNA Synthesis
12. Transcription and RNA Processing
13. Assembly

B. LEARNING OUTCOMES

By the end of this topic, students should be able to:

1. Define viral replication and explain its significance in viral survival and disease causation.

2. Describe the general stages of viral replication, including attachment, penetration, uncoating, replication, assembly, and release.

3. Explain mechanisms of viral attachment and entry into host cells.

4. Differentiate between replication strategies of DNA and RNA viruses.

5. Explain positive-sense and negative-sense RNA virus replication.

6. Describe the role of host cell machinery in viral replication.

7. Explain the synthesis of viral proteins and regulation of early and late gene expression.

8. Differentiate between lytic and persistent viral infections.

9. Relate viral replication mechanisms to cytopathic effects and disease pathogenesis.

10. Apply knowledge of viral replication to diagnosis, antiviral drug targets, and disease control.

C. SUBTOPICS/LESSONS

1. Lesson 1: How the host cell support the replication of viruses
2. Lesson 2: The One-step growth curve
3. Lesson 3: How do viruses carryout their next generation?
4. Lesson 4: Virus attachment (adsorption)
5. Lesson 5 : Virus internalization
6. Lesson 5 (a): Virus internalization (entry by phagocytosis)
7. Lesson 5 (b): Virus internalization (entry by fusion)
8. Lesson 5 (c): Virus internalization (entry by receptor-mediated endocytosis or veropexis)
9. Lesson 6: Virus uncoating
10. Lesson 7: Virus genome replication strategies and gene expression
11. Lesson 8: Virus assembly
12. Lesson 9: Virus maturation
13. Lesson 10: Virus release

D. STUDY QUESTIONS

1. Multiple choice
2. Study questions-1
3. Study questions-2
4. Study questions-3