Topic 14: Laboratory diagnosis of viral infections

A. VIDEOS

1. Laboratory Diagnosis of Viral Diseases -1
2. Laboratory Diagnosis of Viral Infections -2
3. Laboratory diagnosis of viral infections -3
4. Laboratory Diagnosis of viral Diseases -4
5. Laboratory Diagnosis of Viral infections -5
6. Laboratory Diagnosis of viral Diseases -6
7. Laboratory Diagnosis of Viral infections -7
8. Appropriate specimens for maximum viral recovery

B: LEARNING OUTCOMES

By the end of this topic, students should be able to:

1. Define laboratory diagnosis of viral infections and explain its importance in disease management and control.

2. Describe types of clinical specimens used for viral diagnosis and proper methods of collection, storage, and transport.

3. Explain direct and indirect methods used in laboratory diagnosis of viral infections.

4. Describe virus isolation techniques, including cell culture, embryonated eggs, and laboratory animals.

5. Explain antigen detection methods, such as ELISA and immunofluorescence assays.

6. Describe nucleic acid detection techniques, including PCR and real-time PCR.

7. Explain serological methods for detecting antibodies to viral infections.

8. Differentiate advantages and limitations of various diagnostic techniques.

9. Apply appropriate diagnostic methods to specific viral infections in veterinary and medical settings.

10. Appreciate biosafety and quality assurance requirements in viral diagnostic laboratories.

C: SUBTOPICS /LESSONS

1. Lesson 1: Sample collection for diagnosis of viral diseases
2. Lesson2: Specimen shipping and transport storage
3. Lesson 3: Strategies for Cultivation of Viruses
4. Lesson 3: Cultivation of viruses in animals
5. Lesson 3b: Cultivation of viruses in embryonated eggs
6. Lesson 3c: Cultivation of viruses in tissue and cell culture
7. Lesson 3d: Similarities and differences of invitro and in vivo methods for cultivation of viruses
8. Lesson 4: Laboratory methods for the diagnosis of viral diseases
9. Lesson 4a: Visualization of virus particles by using Microscopic techniques
10. Lesson 4b: Detection of viral antigens
11. Lesson 4c: Detection of viral antibodies
12. Lesson 4d: Detection of replicating viruses