BLS 201_Probation_2011

BLS 201: Cell and Tissue Culture Techniques

Question 1:

Describe the “step-by-step” procedures that you would follow in determining the TCID₅₀ of cytomegalovirus that is known to infect monkey kidney (MK-12) cells. Both the virus stock and MK-12 cells are kept differently at -80⁰C              (20 marks)

Question 2:

a) Briefly describe how you would isolate primary epithelial cells from a pig kidney (10 marks)

b) Write short notes on how you would maintain Vero cells (10 marks)

Question 3:

Describe the applications of cell culture in virology (20 marks)

Question 4:

Write short notes on FOUR different virus staining techniques (20 marks)

Question 5:

You have been hired by the Ministry of Health and Social Welfare as a Principal Laboratory Technologists to start a virology cell culture laboratory. Advice your employer on the following:

a) a drawing of the laboratory layout including the placement of major equipment required (10 marks),

b) a list of consumables, supplies and materials required to be purchased and their function (10 marks),