BLS 201_CAT 1_2021

College of Veterinary Medicine and Biomedical Sciences
BLS 201: Cell and Tissue Culture Techniques Date: 15 December 2021 Time: 1 hour

QUESTION 1 (Total 61 marks)
a) What are the three conditions that have to be provided by the incubator for incubation of cell cultures? (6 marks)
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b) By indicating two main possible colour changes of cell culture media, explain why the colour of the media tend to change with time in the cell culture? (8 marks)
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c) Why is the supply of carbon dioxide important in a cell culture laboratory? (5 marks)
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d) Name three functions of cell culture (6 marks)
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e) Briefly explain why understanding the “Do’s” and “Don’ts” of cell culture is important (8 marks)
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f) Provide two reasons as to why cell culture media is important in cell culture? (4 marks)
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g) With two examples explain why buffering systems are important when growing cells in the laboratory (8 marks)
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h) Microorganisms such as bacteria and fungi are enemies of cell culture. Potentiate this statement and provide the main cause of existence of such microorganisms in the laboratory and cell culture (8 marks)
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i) What are the main four conditions and/ or things needed to culture cells in a laboratory? (8 marks)
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QUESTION 2: Mention ONE function of the following items used in cell culture (2 marks each, Total 14 marks)

QUESTION 3: What are the major developments and the names of developers in cell culture technology in the years indicated in the table below? (Total 25 marks)

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