SOKOINE UNIVERSITY OF AGRICULTURE
COLLEGE OF VETERINARY AND MEDICAL SCIENCES
UNIVERSITY EXAMINATION FOR 2rd YEAR BACHELOR OF VETERINARY MEDICINE
DATE: 10th February 2022
TIME: 11:45-2:45 HRS
INSTRUCTIONS: ATTEMPT ALL QUESTIONS
Use well labeled diagrams where necessary
VENUE: CAD LAB & MLT 11
QUESTION ONE (20 Marks)
a) Provide one function of the following equipment and reagents in cell culture; (i) Biosafety cabinet type II, (ii) Vacuum pump, (iii) inverted light microscope, (iv) Freezer, (v) Incubator, (vi) Hemocytometer, (vii) Inorganic salts, (viii) L-glutamine, (ix) Carbohydrates (x) Vitamins (10 marks)
b) Describe how the first cell culture technique in 1905 by Ross Harrison was achieved? (10 marks)
QUESTION TWO (20 Marks)
a) What are the components of Dulbecco’s modified Minimum essential medium (DMEM)? (10 marks)
b) Describe step by step how you would prepare the DMEM in the cell culture laboratory (10 marks)
QUESTION THREE (20 Marks)
Describe the process of preparation of embryo fibroblast and kidney primary cell cultures (20 marks)
QUESTION FOUR (20 Marks)
a) Describe how you would obtain a cell line from a normal organ and an organ with a tumour (14 marks)
b) With one example in each case provide the differences between adherent and suspension cells (6 marks)
QUESTION FIVE (20 Marks)
(a) What are “egg bit” cultures? (10 marks)
(b) How are they used in diagnosis of respiratory viruses? (10 marks)
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