SOKOINE UNIVERSITY OF AGRICULTURE
BLS201 CELL AND TISSUE CULTURE TECHNIQUES
QUIZ 05th November, 2009
1. Give the name of the dye used to stain cells in order to distinguish between
live and dead cells after cell splitting.
2. Name two basal media used in the preparation of cell culture medium.
3. Give one reason why the colour of the basal media is light pink.
4. Name one enzyme used during splitting of adherent cells.
5. Why is EDTA or EGTA added during cell splitting?
6. What is a single passage?
7. Name three conditions that have to be provided by the incubator for
incubation of cell cultures.
8. What does the term “50% confluency” mean?
9. Name two fluorescent dyes that can be conjugated to antibodies.
10. Name a molecule that has a very strong affinity to streptavidin.
11. Name five equipments that can be found in a cell culture laboratory.
12. Name one cryoprotectant that is used for the storage of cells in liquid
nitrogen.
13. What is the temperature of liquid nitrogen?
14. What is the temperature of ultralow deep freezer?
15. What is the temperature of a freezer?
16. What is the temperature of a fridge?
17. Name two reagents that can be used to fix cells.
18. Name one reagent that can be used as a cushion during ultracentrifugation of
viruses.
19. Name two antibiotics that can be included in a cell culture medium.
20. What is the function of HEPES in cell culture medium?
21. Cells were split and 100 μl of the cells suspension were placed onto 900 μl of
cell culture medium. 100 μl of the resulting cell suspension were then added
onto 300 μl of the dye used to distinguish between dead and alive cells. The
average number of cells in a single haemocytometer chamber was 125.
Calculate the number of cells per ml right after splitting.22. Name three functions of cell culture.
23. Name two types of cell lines.
24. How can cells be disintegrated to release intracellular virus?
25. You are provided with FITC‐conjugated anti‐mouse swine antibodies, antiporcine
circovirus mouse antibodies, anti‐porcine parvovirus mouse
antibodies, FITC‐conjugated anti‐porcine mouse antibodies and streptavidinconjugated
anti‐mouse swine antibodies. Name two antibodies that you will
use to stain cells infected with porcine circovirus by indirect
immunofluorescence technique.
26. Name one enzyme that is conjugated to antibodies for use during
immunoperoxidase monolayer assays.
27. How can one wash cells during staining of cells after addition of a primary
antibody?
28. Why is the staining with fluorescent dyes done in the dark?
29. At what temperature can one study virus attachment to cells?
30. Name the microscope used in taking a cross‐section of a cell.