Lesson 3: Laboratory cultivation of viruses

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May 25, 2022

Viruses, unlike living organisms, cannot carry out metabolic processes or replicate on their own. They lack the necessary cellular machinery for growth and reproduction. As a result, viruses rely on host cells to replicate, and their laboratory cultivation involves the use of host organisms.

  1. Tissue Culture:
    • Animal viruses are often cultivated in vitro using tissue culture techniques. Tissue cultures involve growing cells from an organism in a laboratory dish or flask. The cultured cells provide a controlled environment for studying viral infection and replication.
  2. Embryonated Eggs:
    • Some viruses can be cultivated in embryonated eggs, particularly in the case of certain animal and avian viruses. The virus is injected into the developing embryo, and the virus replicates within the embryonic tissues.
  3. Animal Models:
    • In some cases, live animals are used for virus cultivation, particularly for studying the pathogenesis of certain viruses or for testing the efficacy of antiviral drugs or vaccines. However, ethical considerations and the development of alternative methods have reduced the use of animals in virus cultivation.

Laboratory cultivation of viruses is crucial for studying their biology, understanding the mechanisms of infection, and developing antiviral drugs and vaccines. The choice of cultivation method depends on the type of virus being studied and the specific goals of the research.

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